HPLC Determination of Quercetin on Primesep D Column
https://sielc.com/hplc-determination-of-quercetin-on-primesep-d-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method of Quercetin.
Quercetin is a naturally occurring flavonol, or flavonoid, the yellowish antioxidant pigment found in skins of red grapes, apples, berries, onions, tomatoes, and buckwheat tea. In addition to functioning as a flavonoid, quercetin is also a phytoestrogen. It has the chemical formula C 15 H 10 O 7 .
Quercetin can be retained in HPLC using a Primesep D mixed-mode column using a mobile phase of acetonitrile (ACN), water and Trifluoroacetic acid (TFA) buffer. Detection can be achieved with UV 270 nm , mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | TFA – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | 270 nm |
| Class of Compounds | Phenolics compound, Flavonoid |
| Analyzing Compounds | Quercetin |
HPLC Column Used
Primesep D, 4.6 x 150 mm, 5 µm, 100 A, dual ended