HPLC Determination of Prochloraz on Newcrom B Column
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HPLC Determination of Prochloraz on Newcrom B Column_1215
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Prochloraz .
Prochloraz is an imidazole fungicide with the chemical formula C 15 H 16 Cl 3 N 3 O 2 . It is used widely across Europe, Asia, South America, and Australia, but is not registered for use in the United States. It works as an inhibitor of the enzyme lanosterol 14α-demethylase.
Prochloraz can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Prochloraz |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended