HPLC Method for Separation of Procainamide and Procaine on BIST B+ Column Column

Chromatogram

Description

Procainamide is an antiarrhythmic medication with the chemical formula C 13 H 21 N 3 O . It belongs to the Class Ia antiarrhythmic drugs. It is used to treat certain types of abnormal heart rhythms (arrhythmias) by stabilizing the cell membrane and reducing excitability in the heart muscle. Procainamide works by inhibiting sodium channels in cardiac cells, prolonging the action potential duration and refractory period, which helps in controlling irregular heartbeats. It is typically administered intravenously or orally under medical supervision.

Procaine is a local anesthetic drug with the chemical formula C 13 H 20 N 2 O 2 . It belongs to the amino ester group of local anesthetics. It is employed for local anesthesia, providing pain relief in various medical and dental settings. Procaine works by blocking nerve impulses and causing temporary loss of sensation in a specific area of the body. It is usually administered through injection to induce local anesthesia. You can find detailed UV spectra of Procainamide , Procaine and information about its various lambda maxima by visiting the following link.

Procainamide , Procaine can be retained, separated and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a sulfuric acid as a buffer. This analysis method can be detected using UV at 280 nm.

Method Parameters

HPLC Column Used