HPLC Determination of Piracetam on SHARC 1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Piracetam
Piracetam has been marketed as a treatment for myoclonus, vertigo, dyslexia, and sickle cell anemia. It is approved for use across many European countries, but is not approved by the FDA in the United States. It’s chemical formula is C 6 H 10 N 2 O 2 .
[compound] can be detected in the low UV regime. Using a SHARC 1 hydrogen-bonding column and a mobile phase consisting of acetonitrile (MeCN) and water without a buffer, Piracetam can be retained, measured, and analyzed. This method can be UV detected at 200 nm with very high resolution
Method Parameters
| Mobile Phase | MeCN/H2O -98/2% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Drug, Racetam |
| Analyzing Compounds | Piracetam |
HPLC Column Used
SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended