HPLC Determination of Phenethyl alcohol on Newcrom BH Column

https://sielc.com/hplc-determination-of-phenethyl-alcohol
⬇ PDF Download

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Phenethyl alcohol .

Phenethyl alcohol is used in a variety of essential oils and soaps for its pleasant odor and antibiotic properties.

It can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of acetonitrile (ACN) and water with sulfuric acid (H2SO) buffer. UV detection at 200nm.

Method Parameters

Mobile PhaseMeCN – 15%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 200nm
Class of CompoundsHydrophobic
Analyzing CompoundsPhenethyl alcohol

HPLC Column Used

Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended