HPLC Determination of Perindopril on Primesep 200 column
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HPLC Determination of Perindopril
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Perindopril .
Perindopril is an ACE inhibitor used in treating hypertension (high blood pressure). It comes in tablet form. Perindopril can be retained on a Primesep 200 column in under 8 minutes with a simple isocratic mobile phase and sulphuric acid buffer. Primesep 200 is a reverse-phase (RP) mixed-mode column with embedded weak acidic ion-pairing groups
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.4 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 205 nm |
| Class of Compounds | Drug, Acid, Hydrophobic, Ionizable |
| Analyzing Compounds | Perindopril |
HPLC Column Used
Primesep 200