HPLC Determination of Pentadecafluorooctanoic acid (PFOA) on Newcrom R1 Column
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HPLC Determination of Pentadecafluorooctanoic acid on Newcrom R1 Column
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Perfluorooctanoic acid (PFOA) .
Pentadecafluorooctanoic acid , also known as C8 due to it’s chemical being C 8 HF 15 O 2 , is an industrial surfactant. It is classified by the IARC as a carcinogen to humans. Due to it not breaking down via natural processes, it is also considered a “forever chemical.” It can easily move through soil and contaminate water, leading up to it building up[ in fish and wildlife. Despite that, it has wide use across industries from upholstery, to floor wax, to fire fighting sealants. PFOA was a key point in a renowned legal case of Robert Bilott against DuPont.
Perfluorooctanoic acid (PFOA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds | Acid, Hydrophobic |
| Analyzing Compounds | Perfluorooctanoic acid (PFOA) |