HPLC Determination of Octylphosphonic acid on Newcrom A
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Description
Octylphosphonic acid ( OPA ) is a surfactant with the chemical formula C 8 H 19 O 3 P . It can be used to synthesize self-assembled monolayers (SAMs), polymeric matrices, and as a coating for super-resolution microscopy.
Using a Newcrom A mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer, OPA can be retained, measured, and analyzed. This analysis method can be used in conjunction with charged aerosol detection (CAD).
Method Parameters
| Mobile Phase | MeCN/H2O – 25/75% |
|---|---|
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Hydrophobic, Ionizable |
| Analyzing Compounds | Octylphosphonic acid |
HPLC Column Used
Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended