HPLC Determination of Octanoic acid (Caprylic acid) on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Octanoic acid (Caprylic acid) .
Octanoic acid ( Caprylic acid ) is the common name for the eight-carbon straight-chain fatty acid. It can be found in numerous foods such as coconuts, cherries, plums, pineapple sages, black raspberries, and shallots. Octanoic acid is found to be associated with medium-chain acyl-CoA dehydrogenase deficiency, a genetic disorder that affects the metabolism of converting certain fatty acids to energy.
Octanoic acid can be retained on a mixed-mode Primesep 100 column by using an isocratic method consisting of a 50/50% Acetonitrile (ACN) and water mobile phase with a Phosphoric acid (H3PO4) buffer. UV detection at 210 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| LOD* | 7 ppm |
| Class of Compounds | Hydrophobic, Acid |
| Analyzing Compounds | Octanoic acid (Caprylic acid) |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended