HPLC Determination of Mycophenolic Acidon Primesep SB Column
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HPLC Determination of Mycophenolic Acid
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid .
Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60% |
|---|---|
| Buffer | H3PO4- 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 226 nm |
| Class of Compounds | Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
| Analyzing Compounds | Mycophenolic acid |
HPLC Column Used
Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended