HPLC ELSD Method for Analysis of Mogroside V on Primesep AP Column
https://sielc.com/hplc-determination-of-mogroside-vChromatogram
Description
· HPLC Method for Analysis of Mogroside V on Primesep AP by SIELC Technologies
Mogroside V is a natural compound and a type of cucurbitane glycoside with the chemical formula C 60 H 102 O 29 . It is primarily found in the fruit of the luo han guo (Siraitia grosvenorii) plant, also known as monk fruit. Monk fruit has been used in traditional Chinese medicine for its sweetening properties and potential health benefits.
Mogroside V can be retained and analyzed on a Primesep AP mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and acetic acid as a buffer. This analysis method can be detected using ELSD
Method Parameters
| Mobile Phase | MeCN – 80% |
|---|---|
| Buffe | Acetic acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C,with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Sample | 0.7 mg/ml in MeCN/H2O – 50/50% |
| Injection volume | 5 µl |
| Class of Compounds | Bis-biguanide |
| Analyzing Compounds | Mogroside V |
HPLC Column Used
Primesep AP, 4.6 x 150 mm, 5 µm, 100 A, dual ended