HPLC-MS Method for Analysis of Michler’s ketone on Primesep 200 Column
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Description
· Separation type: Liquid Chromatography Mixed-mode · HPLC Method for Analysis of Michler’s ketone on Primesep 100 Column by SIELC Technologies
Michler’s ketone ( 4,4′-bis(dimethylamino)benzophenone ) is a chemical compound used primarily in the synthesis of dyes and pigments. It has the chemical formula C 17 H 17 N 3 O 2 . The compound is named after the German chemist Viktor Michler, who first prepared it.
Michler’s ketone is synthesized by condensing two equivalents of dimethylaniline with one equivalent of phosgene. The reaction involves the formation of a carbonyl group (C=O) that links two dimethylaniline moieties. The resulting structure features two aromatic rings (benzene rings) connected by a carbonyl group, with dimethylamino groups attached to each aromatic ring.
Michler’s ketone is a valuable intermediate in the dye industry, where it is used to prepare various triarylmethane dyes, including Malachite Green, Methyl Violet, and Crystal Violet. The strong electron-donating properties of the dimethylamino groups make it a useful compound for synthesizing dyes with vibrant and stable colors.
Michler’s ketone can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a formic acid as a buffer. This analysis method can be detected in the UV regime at 371 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 371 nm, LCMS SIM 269+ |
| Peak Retention Time | 7.17 min |
| Class of Compounds | Diaryl ketones |
| Analyzing Compounds | Michler’s ketone |