HPLC Method for Analysis of Methyl red on Primesep 100 Column
https://sielc.com/hplc-determination-of-methyl-redChromatogram
Description
· HPLC Method for Separation of Methyl Red on Primesep 100 Column by SIELC Technologies
Methyl Red , also known as C.I. Acid Red 2 , is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions and yellow at solution with pH above 6.2. It has the chemical formula C 15 H 15 N 3 O 2 . You can find detailed UV spectra of Methyl Red and information about its various lambda maxima by visiting the following link.
Methyl Red can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 520 nm |
| Peak Retention Time | 6.19 min |
| Class of Compounds | Dyes |
| Analyzing Compounds | Methyl Red |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended