HPLC Method for Separation of Methyl Red and Ethyl Red on Primesep 100 Column

Chromatogram

Description

· HPLC Method for Separation of Methyl Red , Ethyl red on Primesep 100 Column by SIELC Technologies

Methyl Red , also known as C.I. Acid Red 2 , is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions and yellow at solution with pH above 6.2. It has the chemical formula C 15 H 15 N 3 O 2 . You can find detailed UV spectra of Methyl Red and information about its various lambda maxima by visiting the following link.

Ethyl red is a pH indicator with C 17 H 19 N 3 O 2 as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods. You can find detailed UV spectra of Ethyl Red and information about its various lambda maxima by visiting the following link.

Methyl Red , Ethyl red can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.

Method Parameters

HPLC Column Used