HPLC Determination of Metalaxyl on Newcrom B Column
https://sielc.com/hplc-determination-of-metalaxyl-on-newcrom-b-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Metalaxyl .
Metalaxyl , also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate, is a systemic fungicide with the chemical formula C 15 H 21 NO 4 . It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.
Metalaxyl can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 266 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Metalaxyl |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended