Alltesta HPLC Method for Analysis of Losartan on Newcrom R1 Column
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Description
· HPLC Method for Analysis of Losartan on Newcrom R1 Column by SIELC Technologies
Losartan is a medication with the chemical formula C 22 H 23 ClN 6 O that belongs to a class of drugs known as angiotensin II receptor blockers (ARBs). It is primarily used to treat high blood pressure (hypertension) and certain other conditions related to the heart. It works by blocking the action of angiotensin II, a substance in the body that narrows blood vessels and releases hormones that can raise blood pressure. You can find detailed UV spectra of Losartan and information about its various lambda maxima by visiting the following link.
Losartan can be retained, and analyzed on a reversed-phase Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 200 nm using UV detection
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70%, |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Diphenyl ethers |
| Analyzing Compounds | Losartan |