HPLC-MS Method for Analysis of Lamivudine on Primesep 100 Column
https://sielc.com/hplc-determination-of-lamivudineChromatogram
Description
Lamivudine is an antiviral medication used primarily to treat infections caused by the human immunodeficiency virus (HIV) and hepatitis B virus (HBV). It belongs to a class of drugs known as nucleoside reverse transcriptase inhibitors (NRTIs).
Lamivudine can be retained, and analyzed using a Primesep 100 mix mode stationary phase column. The analysis utilizes a isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium Formate pH 3.0 – 20 mM as a buffer. Detection is carried out using UV, LC-MS.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV at 277 nm; ESI-SIM: [M+H]⁺ 230, 271 |
| Class of Compounds | Drug |
| Analyzing Compounds | Lamivudine |
HPLC Column Used
Primesep 100, 2.1 x 100 mm, 5 µm, 100 A, dual ended