HPLC Determination of Lactic Acid on Newcrom BH Column
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Description
Lactic acid (milk acid) is a natural organic acid that plays an important role in biochemical processes. It has a carboxylic and hydroxy- groups which, upon standing, can react with each other and form dimers, trimers and higher oligomers. Lactic acid is a polar molecule that does not retain on reversed-phase columns. Newcrom BH mixed-mode anion-exchange column is used for the separation of lactic acid and its oligomers by a combination of weak reversed-phase and anion-exchange mechanisms. Lactates can be monitored by low UV, ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | HClO4 – 0.1% |
| Flow Rate | 1 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Lactic Acid,Lactic Acid Oligomers |
HPLC Column Used
Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended