HPLC Method for Analysis of Ketoconazole on Primesep B  Column

Chromatogram

Description

· Method for Analysis of Ketoconazole on Primesep B by SIELC Technologies

Ketoconazole , also known as Nizoral , is an antifungal compound with the chemical formula C 26 H 28 Cl 2 N 4 O 4 . It is used to treat dandruff and is commonly available as a shampoo. It is applied to skin to treat other fungal infections. Ketoconazole also has antiandrogen and antiglucocorticoid properties. It works through interfering with the fungal synthesis of ergosterol and other enzymes. You can find detailed UV spectra of Ketoconazole and information about its various lambda maxima by visiting the following link.

Ketoconazole can be retained and analyzed on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.

Method Parameters

HPLC Column Used