HPLC Method for Separation of Ketoconazole and Potassium Sorbate on Primesep B Column
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Description
Ketoconazole , also known as Nizoral , is an antifungal compound with the chemical formula C 26 H 28 Cl 2 N 4 O 4 . It is used to treat dandruff and is commonly available as a shampoo. It is applied to skin to treat other fungal infections. Ketoconazole also has antiandrogen and antiglucocorticoid properties. It works through interfering with the fungal synthesis of ergosterol and other enzymes. You can find detailed UV spectra of Ketoconazole and information about its various lambda maxima by visiting the following link.
Potassium Sorbate is a popular preservative with the chemical formula C 6 H 7 KO 2 . It is used in a wide variety of foods, wines, and personal-care products. It works through inhibiting molds, yeasts, and various other microbes. You can find detailed UV spectra of Potassium Sorbate and information about its various lambda maxima by visiting the following link.
Potassium sorbate , Sodium sorbate , Ketoconazole can be retained, analyzed, and separated on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-50%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200, 250 nm |
| Peak Retention Time | 6.31, 8.12 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Potassium sorbate,Sodium sorbate,Ketoconazole |