HPLC Method for Analysis of Itraconazole on Newcrom R1 Column
https://sielc.com/hplc-determination-of-itraconazole-2Chromatogram
Description
Itraconazole is an antifungal medication with the chemical formula C 35 H 38 Cl 2 N 8 O 4 . It is used to treat a variety of fungal infections. including but not limited to: Aspergillosis, Blastomycosis, Histoplasmosis, Onychomycosis (fungal infection of the nails), and more. Itraconazole works by inhibiting the synthesis of ergosterol, a critical component of fungal cell membranes. This disruption results in increased cellular permeability, leading to leakage of cellular contents and, ultimately, the death of the fungus.
Uses: It is used to treat a wide range of fungal infections, including:
Itraconazole can be retained, and analyzed on a mixed-mode Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 200 nm using UV detection
Method Parameters
| Mobile Phase | MeCN/H2O – 45%, |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Diphenyl ethers |
| Analyzing Compounds | Itraconazole |