HPLC Method for Analysis of Iodide on Primesep B Column
https://sielc.com/hplc-determination-of-iodideChromatogram
Description
Iodide is an ion with the symbol I – . There is currently a worldwide iodine deficiency, which is a leading preventable cause of intellectual disability, has caused some governments to mandate iodized salt to be sold. It is naturally occurring as a mineral, Iodargyrite. It is essential for thyroid hormone production. Lack of it can lead to iodine deficiency disorders. It is typically found in Iodized salt and occasionally as an antiseptic. You can find detailed UV spectra of Iodide and information about its various lambda maxima by visiting the following link .
Iodide can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 228 nm |
| Peak Retention Time | 4.61 min |
| Class of Compounds | Inorganic |
| Analyzing Compounds | Iodide |