HPLC Determination of Imidazolidinyl urea on SHARC 1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Imidazolidinyl urea .
Imidazolidinyl urea is a preservative with the chemical formula C 11 H 16 N 8 O 8 . used in cosmetics as due to its antimicrobial properties. It is active against a broad spectrum of gram positive and gram negative bacteria, yeast and molds.
Imidazolidinyl urea can be retained in HPLC using a SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The isocratic method uses a mobile phase of acetonitrile (ACN) and water. Imidazolidinyl urea can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN/MeOH – 90/10% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Hydrophilic, Amide, Amine |
| Analyzing Compounds | Imidazolidinyl urea |
HPLC Column Used
SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended