HPLC Method for Separation of Ibotenic Acid and Muscimol on a Primesep 100 Column

Chromatogram

Description

· Method for Separation of Muscimol , Ibotenic acid on Primesep 100 by SIELC Technologies

Ibotenic acid , also known as ibotenate , premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid , is an a -amino acid with the chemical formula C 5 H 6 N 2 O 4 . It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.

Muscimol , also known as agarin , pantherine , and pyroibotenic acid , is a psychoactive GABA-A agonist with the chemical formula C 4 H 6 N 2 O 2 . It is found in Amanita mushrooms. Muscimol is well known for its psychoactive, hallucinogenic, and sedative properties. You can find detailed UV spectra of Muscimol and information about its various lambda maxima by visiting the following link.

Muscimol , Ibotenic acid can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

Method Parameters

HPLC Column Used