HPLC Determination of Hydroxychloroquine on Newcrom A Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Hydroxychloroquine .
Hydroxychloroquine is a prescription medication with the chemical formula C 18 H 26 ClN 3 O . It is used to treat and prevent acute attacks of malaria in adults and children. It is not used in severe or complicated cases. Besides malaria, Hydroxychloroquine is also used to treat discoid lupus erythematosus (DLE), systemic lupus erythematosus (SLE), and chronic rheumatoid arthritis. It is considered unsafe to take this medication alongside other medications.
Hydroxychloroquine can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | AmFm pH 3.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 256 nm, MS-compatible mobile phase |
| Class of Compounds | Hydrophobic, Drug |
| Analyzing Compounds | Hydroxychloroquine |