HPLC Determination of Glyphosine (bis-PMG) on Newcrom B Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosine
Glyphosine is a synthetic plant growth regulator with the chemical formula of C 4 H 11 NO 8 P 2 . It is a colorless liquid that works through decreasing chlorophyll production.
Glyphosine can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | Formic Acid – 3% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Plant growth regulator, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Glyphosine |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended