HPLC Determination of Glyphosate on Newcrom B Column
https://sielc.com/hplc-determination-of-glyphosate-on-newcrom-b-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate .
Glyphosate is a broad-spectrum herbicide. It works through inhibiting the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase. The enzyme is essential for producing amino acids within the plant. Glyphosate is used across agriculture and forestry, as well as rare aquatic environments. It’s chemical formula is C 3 H 8 NO 5 P .
Glyphosate can be retained in HPLC with a Newcrom B mixed-mode column using a mobile phase of acetonitrile (ACN, MeCN), water and phosphoric acid (H3PO4) buffer allowing the use of a UV detector at 200 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H3PO45 – 0.08% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 200 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Glyphosate |
HPLC Column Used
Newcrom B, 3.2 x 100 mm, 5 µm, 100 A, dual ended