HPLC Method for Separation of Glucose and Lysine on Primesep S Column
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Description
· HPLC Method for Separation of Glucose and Lysine on Primesep S Column by SIELC Technologies
Lysine is an essential amino acid typically found in meat, and also cereal grains. It can be manufactured on a large scale by fermenting glucose, one of the most abundant sugars in the world. These two biological compounds can be retained, separated, and analyzed on a normal-phase Primesep S column with a simple isocratic mobile phase consisting of Acetonitrile (MeCN), water (H2O), and Sulfuric acid (H2SO4). This analysis method can be detected via Refraction Index (RI) Detection.
Method Parameters
| Mobile Phase | MeCN/H2O – 75/25% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | Refraction Index |
| Peak Retention Time | 4.81 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Glucose, Lysine |
HPLC Column Used
Primesep S, 4.6×150 mm, 5 µm, 100A