HPLC Determination of Fructose 1,6 Bisphosphate on Newcrom B Column
https://sielc.com/hplc-determination-of-fructose-16-bisphosphateChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Fructose 1,6 Bisphosphate , Phosphate , Fructose 6-Phosphate .
Fructose 1,6-biphosphate , alternatively known as Harden-Young ester , is the main form glucose and fructose take when they enter cells. It is a key component of the glycolysis metabolic pathway and is the product of the phosphorylation of fructose 6-phosphate. It has the chemical formula C 6 H 14 O 12 P 2 . Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile with an ammonium formate (AmFm) buffer, Fructose 1,6-bisphosphate can be retained, separated (from fructose 6-phosphate), and measured. Fructose 1,6 bisphosphate can be detected using charged aerosol detection (CAD) with high resolution.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | AmFm – pH 3.0 – 20 mm |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Sugar Phosphate |
| Analyzing Compounds | Fructose 1,6 Bisphosphate,Phosphate,Fructose 6-Phosphate |