HPLC Determination of Fosfomycin on Primesep SB Column
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HPLC Determination of Fosfomycin
Description
Fosfomycin is a broad-spectrum antibiotic consisting of an epoxide ring and a phosphite group. It can be retained on a Primesep SB column in about 8 minutes using an isocratic MS-compatible mobile phase method. Primesep SB is a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Antibiotic |
| Analyzing Compounds | Fosfomycin |
HPLC Column Used
Primesep SB