HPLC Method for Analysis of Flucytosine on Primesep 100 Column
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Description
Flucytosine , also known as 5-fluorocytosine and 5-FC , is an anti-fungal drug with the chemical formula C 4 H 4 FN 3 O . It is used primarily in tandem with amphotericin B to treat Candina infections and cryptococcosis. It works through being converted to cytostatic fluorouracil, which leads to disturbed and disrupted RNA biosynthesis of certain proteins, and fluorodeocyuridylate which inhibits DNA synthesis of fungi. You can find detailed UV spectra of Flucytosine (5-fluorocytosine, 5-FC) and information about its various lambda maxima by visiting the following link.
Flucytosine (5-fluorocytosine, 5-FC) can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 285 nm |
| Peak Retention Time | 4.81 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Flucytosine (5-fluorocytosine, 5-FC) |