HPLC Method for Analysis of Ethyl Red on Primesep 100 Column
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Description
· HPLC Method for Analysis of Ethyl red on Primesep 100 Column by SIELC Technologies
Ethyl Red is a pH indicator with C₁₇H₁₉N₃O₂ as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods . You can find detailed UV spectra of Ethyl red and information about its various lambda maxima by visiting the following link.
Ethyl red can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 600 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 550 nm |
| Peak Retention Time | 3.82 min |
| Class of Compounds | Dyes |
| Analyzing Compounds | Ethyl red |