HPLC Determination of Escitalopram (Cipralex) on Primesep SB Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Escitalopram .
Escitalopram is a type of antidepressant known as a selective serotonin reuptake inhibitor with the chemical formula C 20 H 21 F N 2 O . It is used to restore serotonergic function in the treatment of depression and anxiety. It is formulated as an oxalate salt. Escitalopram has a lot of side effects that include headache, nausea, and trouble sleeping.
Escitalopram can be retained on Primesep SB , a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. UV detection at 240 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | 240 nm |
| Class of Compounds | Drug, Hydrophobic |
| Analyzing Compounds | Escitalopram |
HPLC Column Used
Primesep SB, 3.2 x 50 mm, 5 µm, 100 A, dual ended