HPLC Determination of EDTA, Phenoxyethanol and Methylparaben in Facial Cleanser on Newcrom BH

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA (Ethylenediaminetetraacetic Acid) , Methylparaben , Methylparaben sodium , 2-Phenoxyethanol .

Ethylenediaminetetraacetic acid (EDTA) is a synthetic amino acid with the chemical formula C 10 H 16 N 2 O 8 . It is typically used in industry to sequester metal ions, which helps prevent change of colors in textiles and uneven bleaching in paper. Due to it being a chelator, it is also used to soften water during laundry, remove hydrogen sulfide from gas streams, as well as treat mercury and lead poisoning.

Phenoxyethanol is a synthetic compound with the chemical formula C 8 H 10 O 2 . It is said to have antimicrobial and preservative properties, leading to wide use of it in cosmetics, medicine, and biocides. It is considered safe in the US and Europe at limited concentrations.

Methylparaben, also known as Methyl 4-hydroxybenzoate, is a paraben with the chemical formula C 8 H 8 O 3 . It is used as a preservative in food, cosmetics, and pharmaceuticals as it is said to have antimicrobial and antifungal properties. It is considered safe for use in low concentrations, but it may cause irritation or contact dermatitis in rare cases and for those who are allergic.

You can find detailed UV spectra of EDTA + Fe complex and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Methylparaben and information about its various lambda maxima by visiting the following link.

EDTA (Ethylenediaminetetraacetic Acid) , Methylparaben , Methylparaben sodium , 2-Phenoxyethanol can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Method Parameters

HPLC Column Used