HPLC Determination of Dursban on Newcrom B Column
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HPLC Determination of Dursban on Newcrom B Column_1216
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorpyrifos-methyl (Dursban) .
Dursban is a highly toxic organophosphate with the chemical formula C 9 H 11 Cl 3 NO 3 PS . It works through interrupting the electrochemical processes in nerves. This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.
Chlorpyrifos-methyl (Dursban) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium Formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Chlorpyrifos-methyl (Dursban) |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended