HPLC Determination of Didodecyldimethylammonium Bromide on Primesep SB Column
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HPLC Determination of Didodecyldimethylammonium bromide on Primesep SB Column_1208
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Didodecyldimethylammonium bromide , Bromide
Didodecyldimethylammonium Bromide is a double-chain cationic surfactant with the C 26 H 56 BrN molecular structure. It is occasionally used as a disinfectant, synthesis of nanoparticles and organoclays. Neuroblastoma and leukemia cells are more sensitive to it than to any other carcinoma cells.
Didodecyldimethylammonium bromide , Bromide can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Formate buffer. Detection is performed using CAD.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | AmFm pH 3.0 – 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Base, Ions, Hydrophobic, Ionizable |
| Analyzing Compounds | Didodecyldimethylammonium bromide,Bromide |