HPLC Determination of Diazepam on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Diazepam .
Diazepam , also known as Valium , is a benzodiazepine. It is thought that benzodiazepines work by enhancing the activity of certain neurotransmitters in the brain. Diazepam increases the activity of gamma-aminobutyric acid (GABA), a special chemical that can send signals throughout the nervous system.
Diazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 240nm.
Method Parameters
| Mobile Phase | MeCN – 30% |
|---|---|
| Buffer | No |
| Flow Rate | 0.2 ml/min |
| Detection | 240 nm |
| Class of Compounds | Drug, Hydrophobic |
| Analyzing Compounds | Diazepam |
HPLC Column Used
Newcrom R1, 2.1 x 100 mm, 5 µm, 100 A, dual ended