HPLC Method Separation of 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) and 1,5-Diaminopentane (Cadaverine) on Primesep 200 Column

Chromatogram

Description

· Separation type: Liquid Chromatography Mixed-mode

1,3-Diaminopropane , or trimethylenediamine , is a diamine with the chemical formula C 3 H 10 N 2 . It is the key heterocycle building block and precursor of the chemotherapy antineoplastic agents Prioxantrone and Losoxantrone. You can find detailed UV spectra of 1,3-Diaminopropane and information about its various lambda maxima by visiting the following link.

1,4-Diaminobutane , or Putrescine , is an organic compound with the chemical formula C 4 H 12 N 2 . It is one of the main sources of the foul odor of rotting flesh. On the industrial scale, it is produced by the hydrogenation of succinonitrile as it has found use as a building block of Nylon 46. It has been proposed to be used as a biochemical marker for determination of how long a corpse has been decomposing. You can find detailed UV spectra of 1,4-Diaminobutane and information about its various lambda maxima by visiting the following link.

1,5-Diaminopentane , or Cadaverine , is an organic compound with the chemical formula C 5 H 14 N 2 . It is a source of the foul odor of rotting flesh and since its produced by the decarboxylation of lysine, can be used as a biomarker for lysine metabolic deficiencies. You can find detailed UV spectra of 1,5-Diaminobutane and information about its various lambda maxima by visiting the following link.

These (putrid) diamines can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

Method Parameters

HPLC Column Used