HPLC Method for Analysis of Dextromethorphan on Primesep 100 Column
https://sielc.com/hplc-determination-of-dextromethorphanChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode · HPLC Method for Analysis of Dextromethorphan on Primesep 100 Column by SIELC Technologies
Dextromethorphan is a cough suppressant that works by affecting the cough reflex in the brain. It is commonly found in the over-the-counter cough and cold medicines. Dextromethorphan can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the low UV regime at 225 and 280 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 225, 280 nm |
| Peak Retention Time | 4.81 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Dextromethorphan |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A