HPLC Determination of Daptomycin on Primesep 100 column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis Daptomycin
Daptomycin is a cyclic lipopeptide antibiotic with the chemical formula C 72 H 101 N 17 O 26 . It displays a dose-dependent killing activity against a broad range of gram-positive bacteria. It has only minimal activity against anaerobic bacteria and no activity against gram-negative bacteria.
Daptomycin can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 45/55% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Daptomycin |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended