HPLC Determination of Cyclopropanecarboxylic Acid on Primesep B Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Cyclopropanecarboxylic Acid .
Cyclopropanecarboxylic Acid is an organic compound with the chemical formula C 4 H 6 O 2 . It is typically used in synthesis of agrochemicals, pharmaceuticals, and other chemical industries. OUtside of synthesis, it is also used in manufacturing of electronics, coating, and adhesives.
Cyclopropanecarboxylic Acid can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Monocarboxylic acid |
| Analyzing Compounds | Cyclopropanecarboxylic Acid |
HPLC Column Used
Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended