HPLC Determination of Cyanoacetic Acid on Primesep SB Column
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HPLC Determination of Cyanoacetic Acid on Primesep SB Column_1187
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Cyanoacetic acid
Cyanoacetic Acid is an organic acid with the chemical formula C 3 H 3 NO 2 . It is used in a variety of syntheses as an intermediate including but not limited to vitamin B6, caffeine, herbicides, pesticides, dyes, and polymers. When heated up, it undergoes decarboxylation to produce acetonitrile.
Cyanoacetic acid can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Cyanoacetic acid |
HPLC Column Used
Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended