HPLC Method for Analysis of Crystal Violet on Primesep 100 Column
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Description
· Separation type: Liquid Chromatography Mixed-mode · HPLC Method for Analysis of Crystal Violet on Primesep 100 Column by SIELC Technologies
Crystal Violet, also known as Methyl Violet 10B, is a synthetic dye belonging to the triarylmethane dye family. It has the molecular formula C25H30ClN3. This dye is primarily known for its use in microbiology as a stain, but it has other applications as well.
Crystal Violet can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis, 590 nm |
| Peak Retention Time | 9.37 min |
| Sample concentration | 0.0006 mg/ml |
| Injection volume | 5 µl |
| LOD | 10 ppb |
| Class of Compounds | Dyes |
| Analyzing Compounds | Crystal Violet |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended