HPLC Determination of Coenzyme Q10 and Ubiquinol on Primesep D Column

Chromatogram

Description

· HPLC Method for Coenzyme q10 , Ubiquinol on Primesep D by SIELC Technologies · High Performance Liquid Chromatography (HPLC) Method for Analysis of Coenzyme q10 , Ubiquinol .

Coenzyme Q10 , also known as ubiquinone , is an enzyme found in animals and is used as a dietary supplement with the chemical formula C 59 H 90 O 4 . It acts as a redox carrier, which means that it transfers electrons in the mitochondria. The production of ubiquinone depletes with age. Ubiquinol is a reduced form of coenzyme Q10.

The two compounds are similar in structure and can be difficult to separate in reverse-phase HPLC. Using mixed-mode Primesep D column and the mobile phase with acetonitrile (ACN) and water, without the need for buffer, CoQ10 and ubiquinol can be isocratically separated and UV detected at 275nm.

Method Parameters

HPLC Column Used