HPLC Determination of Citric Acid on Newcrom B Column
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HPLC Determination of Citric Acid on Newcrom B Column
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Citric Acid
Citric Acid is a naturally occurring organic acid found in citrus fruits; it is also an intermediate in the citric acid cycle of aerobic organisms. It is used industrially as an acidity regulator, flavoring, detergent, and more than 2 million tons are produced annually. It’s chemical formula is C₆H₈O₇ .
Citric Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Citric Acid |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended