HPLC Determination of Ciclopirox on Newcrom A Column
https://sielc.com/hplc-determination-of-ciclopirox-on-newcrom-a-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ciclopirox .
Ciclopirox is a prescription medication with the chemical formula C 12 H 17 NO 2 . It is used to treat topical fungal infections on skin, nails, and scalp. It is available in a variety of forms from shampoo, to nail lacquer, to cream.
Ciclopirox can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 45% |
|---|---|
| Buffer | HClO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Class of Compounds | Drug, Synthetic antifungal agent |
| Analyzing Compounds | Ciclopirox |
HPLC Column Used
Newcrom A, 4.6 x 150 mm, 5 µm, 100 A, dual ended