HPLC Method for Analysis of Cefuroxime on Primesep 100 Column
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Description
Cefuroxime is a cephalosporin antibiotic with the chemical formula C 16 H 16 N 4 O 8 S . It is used to treat a variety of infections including bronchitis, gonorrhea, and Lyme disease. You can find detailed UV spectra of Cefuroxime and information about its various lambda maxima by visiting the following link.
Cefuroxime can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 190, 275 nm |
| Peak Retention Time | 4.81 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Cefuroxime |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended