HPLC Determination of Cefepime on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis Cefepime
Cefepime is a fourth generation cephalosporin antibiotic usually reserved for treatment of severe nosocomial pneumonia and febrile neutropenia as well as multiple drug-resistant microorganisms such as Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae.
Cefepime can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Cefepime |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended