HPLC Determination of Cefadroxil on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis Cefadroxil
Cefadroxil is a cephalosporin antibiotic used to treat many different types of infections caused by bacteria. Some infections that cephalosporins can be used to treat include respiratory tract infections, otitis media (middle ear infection), strep throat, skin infections, skin structure infections, bone infections, and urinary tract infections
Cefadroxil can be retained on the Newcrom BH mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 15/85% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Cefadroxil |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended