HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column
https://sielc.com/hplc-determination-of-carnosine-ps-100Chromatogram
Description
HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column by SIELC Technologies
Carnosine is a dipeptide molecule with the chemical formula C 9 H 14 N 4 O 3 . It is synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer. It is found in muscles and brain tissue, but is produced in the liver. Some use it as a nutritional supplement, but it can be used in topical applications to reduce wrinkles.
Carnosine (beta-alanyl-L-histidine) can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 215 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | TFA – 0.6% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 215 nm |
| Peak Retention Time | 5.21 min |
| Class of Compounds | Phenols |
| Analyzing Compounds | Carnosine (beta-alanyl-L-histidine) |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended